Chloroplast – Definition, Structure, Distribution, function and Diagram

Chloroplasts are specialised organelles found in the cells of green plants and algae. They play a crucial role in the process of photosynthesis, which is essential for plant survival and the maintenance of life on Earth. 

Definition of Chloroplasts

Chloroplasts are double-membraned organelles that contain the photosynthetic pigment chlorophyll. These organelles are responsible for capturing sunlight and converting it into chemical energy through the process of photosynthesis. Chloroplasts are mainly found in the mesophyll cells of plant leaves, where they are highly concentrated.

Chloroplasts are of paramount importance in plants because of their role in photosynthesis. Photosynthesis is a complex biochemical process that converts light energy into chemical energy so that plants can produce organic compounds such as glucose. This process is essential for the synthesis of carbohydrates, which serve as the primary source of energy for plant growth and development.

Chloroplasts also release oxygen as a by-product of photosynthesis, contributing to the oxygen-rich atmosphere that supports life on Earth. The oxygen produced by the chloroplasts is used by the plants themselves, but also by other organisms through respiration.

In addition to energy production, chloroplasts are involved in various other metabolic processes in plant cells. They play a crucial role in the synthesis of amino acids, lipids and other essential molecules that are necessary for the growth and development of the plant.

Also check –Cell Organelles – The Complete Guide

Chloroplast Diagram

Chloroplast Diagram

Structure of Chloroplasts

Chloroplasts are green-coloured plastids present in plant cells that play a crucial role in the process of photosynthesis. They are flat, disc-shaped structures measuring approximately 2-10 mm in diameter and 1 mm in thickness.

Outer Membrane

Chloroplasts have two membranes- the outer membrane and the inner membrane. The outer membrane is the outermost layer of the chloroplast and allows the movement of ions and metabolites in and out of the organelle. It serves as a protective barrier for the chloroplast.

Inner Membrane

The inner membrane is the specialised membrane located within the outer membrane of the chloroplast. It is highly selective and contains transport proteins that facilitate the movement of specific molecules across the membrane. The inner membrane is important for regulating the transport of metabolites and ions within the chloroplast.

Both the outer and inner membranes of chloroplasts are composed of phospholipid layers, each about 6-8 nm thick.

Intermembrane Space

The intermembrane space is the space between the outer and inner membranes of the chloroplast. It has a width of about 10-20 nm. This space acts as a buffer zone and facilitates the exchange of molecules and ions between the cytoplasm and the chloroplast.

Stroma

The stroma is the fluid-filled area inside the chloroplast enclosed by the inner membrane. It is a homogeneous matrix that resembles the cytoplasm of the cell and contains various enzymes, DNA, ribosomes and other substances necessary for chloroplast function. The stroma plays an important role in the dark reactions, also called the Calvin cycle, of photosynthesis, in which carbon dioxide is converted into glucose using the energy obtained from the light reactions.

Thylakoid Membrane

The thylakoid membrane is a system of interconnected membrane sacs called thylakoids or lamellae. These thylakoids are stacked on top of each other to form structures called grana (singular- granum). The thylakoid membranes contain chlorophyll pigments that capture light energy during photosynthesis. They are the main sites for the light-dependent reactions of photosynthesis, in which light energy is converted into chemical energy in the form of ATP and NADPH.

Thylakoid Lumen

The thylakoid lumen is the interior space within the thylakoid sacs. It is filled with an aqueous solution and is separated from the stroma by the thylakoid membrane. The thylakoid lumen plays a crucial role in the movement of protons (H+) during the process of photosynthesis.

Grana

Grana are stacks of thylakoid sacs held together by proteins. They are the sites where the thylakoid membranes are densely packed with chlorophyll pigments. The grana serve to increase the surface area available for capturing light energy during photosynthesis. Each granum typically contains about 10-20 thylakoid sacs.

Lamellae

Stroma lamellae, also known as intergranal lamellae, are flattened membrane compartments that connect the stacks of thylakoid sacs or grana. They extend from one granum to another and form a network within the stroma. The lamellae increase the efficiency of photosynthesis by facilitating the transfer of molecules and maintaining structural integrity within the chloroplast.

Chlorophyll

Chlorophyll is a green photosynthetic pigment found in the thylakoid membranes of chloroplasts. It plays a crucial role in capturing light energy during photosynthesis. Chlorophyll molecules absorb light in the blue and red regions of the electromagnetic spectrum and reflect green light, giving chloroplasts their characteristic green colour. This absorbed light energy is then converted into chemical energy that drives the synthesis of ATP and NADPH in the thylakoid membranes and starts the process of photosynthesis.

Distribution of Chloroplasts- Where Are Chloroplasts Found ?

Chloroplasts are mainly found in the cells of plant species, especially in the green parts of the plants where photosynthesis takes place. Here is a detailed description of the distribution of chloroplasts in different plant tissues and cells-

Leaf Mesophyll Cells

Chloroplast - Definition, Structure, Distribution, function and Diagram
Chloroplasts in Leaf Mesophyll Cells

In plants, chloroplasts are mainly found in the mesophyll cells of the leaves. Mesophyll cells are specialised in photosynthesis and contain a high density of chloroplasts. These cells are found in the inner tissue of the leaves where they are exposed to sunlight and can efficiently capture light energy for photosynthetic processes.

Stem Parenchyma Cells

Chloroplasts are also present in the parenchyma cells of the stems. Parenchyma cells are versatile and involved in various functions, including photosynthesis. Stem tissue does not have as many chloroplasts as leaves, but may still contain chloroplasts in cells exposed to light.

Petal Cells

In some plant species, chloroplasts are found in petal cells of flowers. Petals are responsible for attracting pollinators and may contain chloroplasts to carry out photosynthesis, especially in species with green coloured petals.

Green Algae and Aquatic Plants

Chloroplasts are not restricted to land plants. They are also present in various species of green algae and aquatic plants. These organisms have chloroplasts in their cells to facilitate photosynthesis so that they can thrive in an aquatic environment.

Non-Photosynthetic Plant Organs

While chloroplasts are primarily associated with photosynthetic organs, they may also be present in smaller amounts in non-photosynthetic plant organs such as roots and fruits. However, the number and functionality of chloroplasts in these organs are usually limited compared to photosynthetic tissues.

It is important to note that chloroplasts are not present in all organisms. They are only present in photosynthetic organisms, especially plants and some algae. Non-photosynthetic organisms, including animals and fungi, do not have chloroplasts. These organisms obtain their energy in other ways, for example by consuming organic material or by chemosynthesis.

Chloroplasts are found mainly in the cells of photosynthetic plant tissue, including the mesophyll cells of leaves, the parenchyma cells of stems and petal cells. They are also present in green algae and aquatic plants. However, chloroplasts are not present in non-photosynthetic organisms, which underlines their role in the process of photosynthesis and their importance in plant metabolism.

Also Check – Parts of Plant Cell – Location , Structure and Functions

Function of Chloroplasts

Chloroplasts perform essential functions in plant cells, mainly related to the process of photosynthesis and the synthesis of organic compounds. Here are the main functions of chloroplasts-

  1. Photosynthesis

The most important function of chloroplasts is to carry out photosynthesis, the process by which plants convert light energy into chemical energy. Chloroplasts contain chlorophyll, a pigment that absorbs light energy from the sun. This absorbed energy is used to power the synthesis of organic molecules, particularly glucose, which serves as the primary energy source for the plant.

  1. Conversion of light energy

Chloroplasts absorb light energy over a range of wavelengths and convert it into chemical energy through a series of complex reactions. The captured light energy is used to synthesise ATP (adenosine triphosphate), the cell’s primary energy currency, and NADPH (nicotinamide adenine dinucleotide phosphate), an electron transfer molecule involved in various metabolic processes.

  1. Chlorophyll Function

In chloroplasts, chlorophyll molecules are responsible for capturing light energy and making it usable. These pigment molecules are located in the thylakoid membranes of the chloroplasts. They absorb photons of certain wavelengths, especially in the blue and red regions of the spectrum, while reflecting green light, which gives chloroplasts their characteristic green colour. The absorbed light energy is then converted into chemical energy through a series of photochemical reactions.

  1. Oxygen production

During photosynthesis, chloroplasts release oxygen as a by-product. In the thylakoid membranes, water molecules are split by a process known as photolysis, releasing molecular oxygen (O2) into the atmosphere. This oxygen is essential for aerobic respiration in living organisms and for maintaining the balance of atmospheric gases.

  1. ATP Generation

Through the process of photosynthesis, chloroplasts produce ATP, a molecule that serves as a universal energy source in cells. The light-dependent reactions that take place in the thylakoid membranes generate ATP, which is used in various cellular processes, including biosynthesis, active transport and enzyme-catalysed reactions.

  1. Carbon Fixation

Chloroplasts are involved in carbon fixation, the conversion of inorganic carbon dioxide (CO2) from the atmosphere into organic carbon compounds. During the Calvin Cycle, also known as the dark reaction or light-independent reaction, carbon dioxide is combined with the energy-rich molecules ATP and NADPH produced in the light-dependent reactions. This process leads to the formation of glucose and other organic molecules that serve as building blocks for plant growth and metabolism.

Frequently Asked Important questions on Chloroplast

What is the primary function of chloroplasts in plants?

Answer- The primary function of chloroplasts in plants is to carry out the process of photosynthesis, which converts light energy into chemical energy. This process allows plants to produce organic compounds, such as glucose, and is essential for plant growth and development.

What is the structure responsible for capturing light energy during photosynthesis?

Answer- The thylakoid membranes, specifically the chlorophyll pigments within them, are responsible for capturing light energy during photosynthesis.

Also Check – 16 Differences Between Plant Cell and Animal Cell

What is the role of the stroma in chloroplasts?

Answer- The stroma is the fluid-filled region inside the chloroplast. It contains various enzymes, DNA, ribosomes, and other substances necessary for the functioning of the chloroplast. The stroma plays a vital role in the dark reactions (Calvin cycle) of photosynthesis, where carbon dioxide is converted into glucose using the energy derived from the light reactions.

What is the function of the grana in chloroplasts?

Answer- The grana are stacks of thylakoid sacs held together by proteins. They serve to increase the surface area available for capturing light energy during photosynthesis. The thylakoid membranes within the grana contain chlorophyll pigments that capture light energy and initiate the light-dependent reactions of photosynthesis.

Which pigment gives chloroplasts their characteristic green colour?

Answer- Chlorophyll is the pigment responsible for giving chloroplasts their characteristic green colour. Chlorophyll molecules absorb light in the blue and red regions of the electromagnetic spectrum, while reflecting green light.

What is the by-product of photosynthesis released by chloroplasts?

Answer-The by-product of photosynthesis released by chloroplasts is oxygen. During photosynthesis, oxygen is produced as a result of splitting water molecules, and it is released into the atmosphere.

Name two other metabolic processes in which chloroplasts are involved.

Answer- In addition to photosynthesis, chloroplasts are involved in the synthesis of amino acids, lipids, and other essential molecules necessary for the growth and development of the plant.

Also Check – Absorbing Facts About Chloroplast You May Not Have Known Before

What is the significance of the intermembrane space in chloroplasts?

Answer- The intermembrane space acts as a buffer zone between the outer and inner membranes of the chloroplast. It facilitates the exchange of molecules and ions between the cytoplasm and the chloroplast, contributing to the proper functioning of the organelle.

What is the role of the thylakoid lumen in photosynthesis?

Answer-The thylakoid lumen is the interior space within the thylakoid sacs. It plays a crucial role in the movement of protons (H+) during the process of photosynthesis. The movement of protons across the thylakoid membrane is essential for the generation of ATP, a molecule that stores and transports energy within the cell.

What are the dimensions of chloroplasts?

Answer-Chloroplasts are typically flat, disc-shaped structures measuring approximately 2-10mm in diameter and 1 mm in thickness.

In which cells of plants are chloroplasts predominantly found?

Answer- Chloroplasts are predominantly found in the mesophyll cells of leaves, which are specialised for photosynthesis and contain a high density of chloroplasts.

Apart from leaves, name another plant tissue where chloroplasts are present.

Answer- Chloroplasts are also present in the parenchyma cells of stems, although in lower quantities compared to leaves.

In which part of a flower can chloroplasts be found?

Answer-In some plant species, chloroplasts can be found in the petal cells of flowers, especially in species with green-coloured petals.

Are chloroplasts exclusive to land plants, or are they found in other organisms as well?

Answer- Chloroplasts are not limited to land plants. They are also present in various types of green algae and aquatic plants.

Do non-photosynthetic plant organs, such as roots and fruits, contain chloroplasts?

Answer- Non-photosynthetic plant organs may contain chloroplasts in limited quantities, but the number and functionality of chloroplasts in these organs are typically lower compared to photosynthetic tissues.

Which pigment within chloroplasts is responsible for absorbing light energy during photosynthesis?

Answer- Chlorophyll is the pigment within chloroplasts that absorbs light energy during photosynthesis.

What is the primary function of chloroplasts in plant cells?

Answer- The primary function of chloroplasts is to carry out photosynthesis, converting light energy into chemical energy and synthesising organic compounds, particularly glucose, which serves as the plant’s primary source of energy.

Besides glucose, name another molecule produced by chloroplasts during photosynthesis.

Answer- Chloroplasts produce oxygen as a byproduct of photosynthesis through the process of splitting water molecules.

What is the role of ATP in cellular processes?

Answer- ATP serves as a universal energy carrier in cells. It is produced by chloroplasts during photosynthesis and is utilised in various cellular processes, including biosynthesis, active transport, and enzyme-catalysed reactions.

What is the process called by which chloroplasts convert inorganic carbon dioxide into organic carbon compounds?

Answer-The process is called carbon fixation, and it occurs during the Calvin Cycle or the dark reaction/light-independent reaction of photosynthesis.

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