Tissue Culture Class 10

What is Tissue culture? 

Tissue culture can be defined as the process of growing living cells artificially in the laboratory .

 In this process plants are grown by asexual propogation by using living

Tissues in artificial media under sterile condition . Plant tissue are nourished by nutrients, hormones, and light . It is also called micropropagation.

Steps of Tissue culture

  1. Small pieces are cut from the plant like flower buds, stem tissue, growing tips, leaves, etc. 
  2. They are then transferred to an artificial nutrient medium where they divide rapidly to form a callus.
  3. This callus is then transferred to a fresh medium containing hormones for growth and differentiation, where it subsequently develops into plantlets.
  4. The new plantlets  are placed in soil to grow into a mature plant.

Importance of Tissue culture

  • There are many advantages of tissue culture over other methods of plant propagation.
  • It is possible to produce a large number of plants from a single parent  in relatively small space and in a short period of time.  
  • The tissue culture process  reduces growing space, labor, and plant maintenance requirements.
  • Viruses and other plant diseases are eliminated by propagating the quickly dividing cells of the shoot tip.
  • Rapid production of ornamental plants like carnations

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